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警告：
WARNING NOTICE:
本課程所介紹的實驗具潛在地危險並需完整地安全訓練、特別的設備及儀器，並需有適當的人員於一旁監督。你必須為實施這些安全措施及方法承擔唯一的責任、義務及危險性。MIT無須為所提供的教材或實施所提供的資料擔負任何責任、義務及危險性。
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
法律聲明
Legal Notice
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以下為學生於本課程所使用之實驗方法的索引，可以pdf格式下載。
The following is an index of laboratory protocols, downloadable in pdf form, that are used by students in this course.
實驗記錄之指引與建議
Guidelines and Recommendations for Lab Notebooks
實驗記錄是良好實驗操作的關鍵。它必須完整的記錄所有實驗的確實操作過程。一份好的實驗記錄可幫助一個人在一段時間後確實的重建當時的實驗。
The notebook is the key to good experimental practice. It should be a complete record of all the experiments as they were actually performed. A good notebook will enable someone to reconstruct, long after the fact, exactly what was done and why.
實驗記錄應標有頁數。於實驗進行時你應確實記錄。實驗結果、表、圖以及相片應直接記錄或黏貼於記錄本作為永久保存。
Laboratory notebooks should be bound with numbered pages. In the notebook you should write down, as you go along, what was actually done. Experimental results, tables, graphs, and photographs should be written directly in your notebook or taped to the pages for a permanent record.
你的實驗記錄至少應包括下列資料：
Your notebook should minimally include the following information:
- 目錄
- Table of contents
在記錄本的開頭預留數頁給目錄。目錄應記錄實驗的短標題、次數與日期。
Leave extra pages at the beginning of the notebook for a table of contents, which you'll add as you go along. The table of contents should have a short title of each experiment, numbered and dated.
- 實驗的簡短原由
- A short summary of the reasoning for the experiment
通常一句就夠。你可能會想涵蓋所用實驗方法的摘要。
Often a single sentence will suffice. You may want to include a summary of the method you will use.
- 所有關於準備實驗的相關資訊
- All the relevant information about the preparation of the experiment
這可大部分藉由適當地交叉引用之前的實驗而達成。當第一次提到某個解決方案時，必須註明出處。如果某個成分或方法第一次使用時，此時必須詳細描述其內容。對於菌種、質粒也是如此。
Much of this can be accomplished by suitable cross references to previous experiments. The first time a solution is introduced, its origin should be noted. If a component or method is used for the first time, then it should be described in detail. The same should apply to bacterial strains, plasmids, etc.
- 實際操作過程
- The actual series of events as performed
在此特別重要的是實際孵育的時間（有異於原本計劃的）、任何流程的改變、任何不尋常的觀察等。寫下實際所發生的事，即使有任何錯誤發生，使我們得以解釋一個實驗。
Especially important here are the actual times of incubation (as opposed to what was planned), any changes in procedure, any unusual observations made, etc. Writing down what actually happened can make it possible to interpret an experiment even though many mistakes were made.
- 結果
- The results
包括最初所收集之原始數據。例如，記錄實際發現之菌落數，以及由此所計算出之細胞濃度。一份簡短的結果與解釋的摘要亦可幫助日後回顧這些資料。
Include raw data in the form in which it was collected. For example, record the actual number of colonies found, as well as the cell concentration calculated from this number. A brief summary of the results and interpretation will also be helpful when reviewing the data at a later date.
重點是只憑藉記憶是不明智的。教師們鼓勵你直接寫在實驗記錄中並且不要於事後浪費時間把它抄進去。
The main point is that it is unwise to rely solely on memory. The staff urges you to write directly into your notebooks and not to waste time copying things down later.
有經驗後，你可能會想了解一些關於某個當時看來不甚重要的實驗，如果你於課程進行中能養成記錄的習慣，那麼由先前的實驗中學習到東西的機會將會大增。
After acquiring some experience, you may want to know something about an early experiment which did not seem important at the time. If you are in the habit of writing things as you go along, the chance of being able to learn from the early experiments is much greater.
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