|
|
|
|
|
» 首頁 » Microbiology » 課堂講稿
|
|
|
|
The Lab Exercises have been created over many years as a team effort by the Molecular Biology and Microbiology faculty.
1. Date and Time:
Fall 2004, 2 hours
2. Throat flora exercise
Conclude study of throat flora. Prepare a brief report
listing the bacteria identified and the test results supporting your
conclusions. (Lab Results, last page)
3. Isolation of
Staphylococcus
3.1. Special Materials:
Examples of S. aureus and
S. epidermidis plates with antibiotic discs, rulers.
3.2. Procedures:
Read antibiotic sensitivity plates. Use the rulers to
measure the diameter of the zone of growth inhibition. Use the following table
to determine if your isolates are susceptible to a particular
antibiotic.
| Zone Diameter
Interpretive Table |
| 嚜� |
|
Diameter of Inhibition zone
(mm) |
| Antimicrobial Agent |
Disc contents |
Resistant |
Intermediate or Indeterminant |
Moderately Susceptible |
Susceptible |
| Ampicillina (AM) |
10繕g |
�楆13 |
- |
14-16 |
�竉17 |
| Cefoxitin
(FOX) |
30繕g |
�楆14 |
- |
15-17 |
�竉18 |
| Clindamycin
(CC) |
2繕g |
�楆14 |
15-20 |
- |
�竉21 |
| Erythromycin
(E) |
15繕g |
�楆13 |
14-22 |
- |
�竉23 |
| Gentamicin
(GM) |
10繕g |
�楆12 |
13-14 |
- |
�竉15 |
| Penicillin
Gb
|
10 U |
�楆28 |
- |
- |
�竉29 |
| Penicillin
Gc
|
10 U |
�楆14 |
- |
�竉15 |
- |
| Vancomycin
(VA) |
30繕g |
�楆9 |
11-Oct |
- |
�竉12 |
|
aGram-negative enteric organisms |
bStaphylococci
|
cEnterococci
|
4. Fecal flora
4.1. Special Materials:
Nutrient agar plate, antibiotic discs,
forceps, soft agar tubes with glucose, lactose and maltose, example of E.
coli and Salmonella on MacConkey lactose plates
4.2. Procedure:
- Inspect MacConkey plates for growth and colony color.
Also, note the relative number of colonies present.
- Score plates for tetracycline and ampicillin resistance.
- Gram stain colonies.
- Determine antibiotic sensitivity profile of a
Lac+, Tetr or Lac+,
Ampr clone. Use the same method you did for Staphylococcus
aureus (fourth lab).
- The use of sugar fermentation tests facilitates
identification of bacteria. Sets of tubes containing soft agar, a pH indicator,
and various sugars are provided. The sugars are glucose, lactose, and maltose.
Inoculate one tube of each sugar with a Lac+ colony. These tubes
will be incubated for one or two days at 37簞C.
BEFORE LEAVING, PUT AWAY EVERYTHING AND CLEAN
BENCHES AND THEN YOUR HANDS !!!
|
|
