此部份的課程介紹包括熟悉一系列化學實驗技巧的單元。創意研究主題作業的資訊及技術指導的清單也會提供。所有的材料可以在完整的實驗指南中找到。
(英文PDF)、 (英文DOC)

我們推薦以下《數位實驗技巧指南》(DLTM)中的教學影片，可用以補充某些單元。

實驗介紹的文件包含安全指導方針，和本課check in和check out的步奏。
(英文PDF)、 (英文DOC)

This section includes course modules for mastering a series of chemistry laboratory techniques. Information on the original research project assignment and a listing of techniques guides are also provided. All materials may be found in the complete laboratory manual. (PDF)

Video instruction tutorials from the Digital Lab Techniques Manual (DLTM) are recommended for some of the modules and are noted below.

The Laboratory Introduction (PDF) document reviews safety guidelines as well as check in and check out procedures for the course.

閱讀
Introductory Reading Assignment

技術單元
Technique Modules

創新研究主題
Original Research Project

技術簡介
Technique Guides

講義
Handouts


閱讀作業介紹
Introductory Reading Assignment

在我們開始實驗之前，內容中有許多章節你必須先閱讀。我們在實驗室的時間是有限的，所以將會分秒必爭，因此要你準時閱讀完是很重要的。在5.301中，必要的閱讀不只會使你成功且將會幫助你成為實驗型化學家。

所以，在你開始你的實驗前，用一些時間來閱讀下述在Zubrick中的一些章節。此課文被選出是因為它很容易閱讀並非常實用。為了更進一步的閱讀此主題或相關主題，我們建議由Leonard, Lygo和 Procte所選出來的課文清單。從科學圖書館的藏書室中有這本課本。想要徹底的將實驗步驟了解而只靠簡單的閱讀通常是很困難的，但是利用簡介的閱讀方式，實驗室的練習，及實驗後的回顧閱讀，你將會記得大部份我們在5.301中所學的。

在每一個實驗階段的開始，將會有一個當天主題的簡短概述，由Tabacco博士和你的助教們協助你們的閱讀作業的討論和實際的實驗室實驗。你也可以看《數位實驗技巧指南》中的有相關的部份以給你重要技巧的實際操作。如果你有閱讀作業上的問題，我們也將會留時間來回答問題。

下列清單是此課程大多數的閱讀作業。在IAP期間將會有額外的閱讀作業，但此介紹性的閱讀是為了讓你熟悉典型的化學實驗室。

Zubrick, James　《有機化學生存指南:學生技巧簡介》第六版
(第一章：安全、第二章：筆記本、第四章：硬體組裝、第六章：有趣的裝置、 第九章：清潔及乾燥、第十章：乾燥劑、第十一章：產物、第十五章：萃取與沖洗、第十八章：加熱、第十九章：夾子、第三十一章：實驗室的儀器使用)

Leonard, Lygo, 和 Procter：《進階實用有機化學》第二版
(第一章：簡介；第二章：安全、第三章：記錄保存、第四章：實驗室裝置、第八章：真空幫浦)

Before we get started in the lab, there are several chapters in the text that you must read first. Our time in the lab will be intense, but of limited duration, so it is important that you complete the readings on time. Not only is the reading essential to your success in 5.301, but it also will help in your development as an experimental chemist.

So, before you begin your experiments, take some time to read over the following chapters in Zubrick. This text was selected because it's easy to read and very practical. For more in-depth reading on these and related topics, we recommend the listed selections in the text by Leonard, Lygo, and Procter. A copy of this text will be available in the Reserve Book Room of the Science library. It is often difficult to fully grasp a laboratory concept by simply reading about it, but using the strategy of introductory reading, practicing in the lab, and post-lab review reading you will retain most of what we will cover in 5.301.

At the beginning of each lab period, there will be a short overview of that day's topic where Dr. Tabacco and your TAs will facilitate a discussion of the assigned reading and the actual lab experiment. You will also view relevant portions of The Digital Lab Techniques Manual to give you a visual representation of the important techniques. Time will be set aside to answer questions that you have from the readings.

The following list is the bulk of the reading for the course. There will also be additional reading during IAP, but this introductory reading is meant to familiarize you with the typical chemistry laboratory.

Zubrick, James. The Organic Chem Lab Survival Manual: A Student's Guide to Techniques. 6th ed.
(Chapters 1- Safety; 2- Notebooks; 4- Jointware; 6- Interesting Equipment; 9- Clean and Dry; 10- Drying Agents; 11- On Products; 15- Extraction and Washing; 18- Heat; 19- Clamps; 31- Instrumentation in the Lab)

Leonard, Lygo, and Procter. Advanced Practical Organic Chemistry. 2nd ed.
(Chapters 1- Introduction; 2- Safety; 3- Keeping Records; 4- Equipping the Lab; 8- Vacuum Pumps)


技術單元
Technique Modules

2.6的重要部份是涵蓋技術單元以致於組成課程的大部份。在此處的五大主題是轉移與萃取、再結晶純化、蒸餾純化、快速層析法純化和蛋白質測定與誤差分析。很重要的一點是實驗指南並不會提供你完成此實驗所需的資訊。許多重要的資訊你將會在預讀作業中找到，而有一些將會在實驗前簡短概述中討論。此三條路(課文、指南和討論)將會讓你準備好來對付技術單元中的實驗要點。

2-6節很重要的部份是技術清單。每一節的開始列出你在實驗期間將會遇到的技術。當你完成的技術單元，你將要回到技術核對清單並在清單上標記以證明你很熟練。如果你對特殊的技術仍然不熟練，那麼你將要多練習幾次直到你覺得有信心可以去運用於不同的實驗中。此了純化與操作技巧外，此節也將會介紹給你光譜技術像是核磁共振光譜(NMR)、紅外光譜(IR)、氣相層析法(GC)和紫外光-可見光(UV-Vis)光譜。

Sections 2–6 cover technique modules that make up the bulk of the class. The five topics included here are "Transfer and Extraction," "Purification by Crystallization," "Purification by Distillation," "Purification by Flash Column Chromatography," and "Protein Assays and Error Analysis." It is important to note that the manual does not contain all of the information that you will need to complete these experiments. Some important information will be found in your pre-lab reading, while the rest will be covered during the pre-lab discussion. This three-pronged approach (the texts, the manual, and the discussions) will prepare you to tackle the experiments outlined in the technique modules.

An important part of sections 2-6 is the techniques checklist. Each section begins with a list of techniques that you will encounter during the experiment. When you have completed a technique module, you should return to the techniques checklist and check off all of the techniques that you have mastered. If you are still uncomfortable with a specific skill then you should practice it until you feel confident that you could apply it in a different experiment. In addition to various purification and manipulation techniques, this section will also introduce you to spectroscopic techniques like nuclear magnetic resonance (NMR) spectroscopy, infrared (IR) spectroscopy, gas chromatography (GC), and ultraviolet-visible (UV-Vis) spectroscopy.

Note:

「CC」是指「合格的化學家」
"CC" refers to the "Competent Chemist" modules.
「EE」是指「有經驗的實驗者」
"EE" refers to the "Expert Experimentalist" modules.

創新研究計畫(PDF)
Original Research Project (PDF)

在實驗指南的第七部份中，這些簡短的實驗的將會介紹給你一些實際的創新研究並示其刺激性。
Found in section seven of the Lab Manual, this brief set of experiments will introduce you to some of the realities and excitement of performing original research.


技術簡介
Technique Guides

在實驗指南的第八部份中，這些簡介將會提供你一些在化學實驗室更常碰到的技術並一步一步的指導你。
Found in section eight of the Lab Manual, these guides will provide you with step-by-step instructions for some of the more common techniques encountered in a chemistry laboratory.

講義
Handouts

光譜技術介紹(PDF)
Introduction to Spectroscopic Techniques (PDF)

數位實驗技巧指南(PDF)
The Digital Lab Techniques Manual (PDF)


致謝
Acknowledgements

此課程的實驗指南和材料是由Katherine J. Franz and Kevin M. Shea 和助理教授Rick L. Danheiser 和Timothy M. Swager。材料則由J. Haseltine, Kevin M.Shea和Sarah A. Tabacco所校訂。
The laboratory manual and materials for this course were prepared by Katherine J. Franz and Kevin M. Shea with the assistance of Professors Rick L. Danheiser and, and Timothy M. Swager. Materials have been revised by J. Haseltine, Kevin M.Shea, and Sarah A. Tabacco.