7.340 Nano-life: An Introduction to Virus Structure and Assembly, Fall 2005
Structure of the Salmonella bacteriophage epsilon15 as determined by a symmetry-free reconstruction of an 11,000 particle dataset obtained by cryo-electron microscopy. An icosahedral shell consisting of 415 copies of capsid protein encloses a 39.7 kilobase dsDNA genome. This reconstruction method also allows the visualization of the complex of proteins that make up the phage's tail. The club like appendages recognize and bind lipopolysaccharide on the surface of Salmonella and anchor the phage to its host during the first steps of infection. (Image kindly provided by Dr. Wen Jiang, Purdue University. Used with permission.)
課程重點
This course features a complete bibliography of readings and sample student assignments in the assignments section.
課程描述
Watson and Crick noted that the size of a viral genome was insufficient to encode a protein large enough to encapsidate it and reasoned, therefore that a virus shell must be composed of multiple, but identical subunits. Today, high resolution structures of virus capsids reveal the basis of this genetic economy as a highly symmetrical structure, much like a geodesic dome composed of protein subunits. Crystallographic structures and cryo-electron microscopy reconstructions combined with molecular data are beginning to reveal how these nano-structures are built. Topics covered in the course will include basic principles of virus structure and symmetry, capsid assembly, strategies for enclosing nucleic acid, proteins involved in entry and exit, and the life cycles of well understood pathogens such as HIV, influenza, polio, and Herpes. A review of cutting edge structural methods is also covered.
