7.340 2005秋季课程：纳米生命：病毒与装配导论(Nano-life: An Introduction to Virus Structure and Assembly, Fall 2005)
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沙门菌属蚀菌体epsilon 15的结构作为确定一个对称性再生成的例子，使用对一个11，000粒病毒颗粒获得的低温电子显微技术数据集。 一个廿面体病毒壳体由415个病毒衣壳蛋白质拷贝组成，包围着一个39.7千个碱基对双股脱氧核糖核酸基因组。这个重新构建方法也允许对组成噬菌体尾部的蛋白质复合体进行检验。这个类似于俱乐部的在沙门菌属表面附属设备认定和脂多糖粘合会粘连细菌噬菌体到它的宿主在侵染的第一个阶段中。（图片由来自普渡大学Wen Jiang博士友情提供。）
Structure of the Salmonella bacteriophage epsilon15 as determined by a symmetry-free reconstruction of an 11,000 particle dataset obtained by cryo-electron microscopy. An icosahedral shell consisting of 415 copies of capsid protein encloses a 39.7 kilobase dsDNA genome. This reconstruction method also allows the visualization of the complex of proteins that make up the phage's tail. The club like appendages recognize and bind lipopolysaccharide on the surface of Salmonella and anchor the phage to its host during the first steps of infection. (Image kindly provided by Dr. Wen Jiang, Purdue University. Used with permission.)
Structure of the Salmonella bacteriophage epsilon15 as determined by a symmetry-free reconstruction of an 11,000 particle dataset obtained by cryo-electron microscopy. An icosahedral shell consisting of 415 copies of capsid protein encloses a 39.7 kilobase dsDNA genome. This reconstruction method also allows the visualization of the complex of proteins that make up the phage's tail. The club like appendages recognize and bind lipopolysaccharide on the surface of Salmonella and anchor the phage to its host during the first steps of infection. (Image kindly provided by Dr. Wen Jiang, Purdue University. Used with permission.)
课程重点
这课程包括一份完整的相关阅读资料书目（PDF）和在作业部分的示例学生作业。
This course features a complete bibliography of readings and sample student assignments in the assignments section.
This course features a complete bibliography of readings and sample student assignments in the assignments section.
课程描述
Watson和Crick注意到以一个病毒的基因组是不足以编码一个大到足以包裹它自身的蛋白质的，并且评论说，一个病毒的壳体必然是由多样的、但相同的二级单位组成。今天，高分辨率的病毒衣壳结构揭示出这遗传的经济性的基础是一个高度对称的结构，非常类似于一个由蛋白质二级单位组成的测地线状的穹丘。结晶学的结构和低温－电子显微技术再生结合随着分子的数据是正在开始揭示这些纳米－结构是如何被组成。本课程涉及的专题包括病毒结构和对称性基本原理，衣壳组合件，封入核酸的策略，蛋白质参与的引入和引出，为人熟知的病源比如HIV（编注：人体免疫缺损病毒），流形性感冒，骨髓灰质炎和疱疹等的生命周期。我们也将回顾切割边缘结构方法。
Watson and Crick noted that the size of a viral genome was insufficient to encode a protein large enough to encapsidate it and reasoned, therefore that a virus shell must be composed of multiple, but identical subunits. Today, high resolution structures of virus capsids reveal the basis of this genetic economy as a highly symmetrical structure, much like a geodesic dome composed of protein subunits. Crystallographic structures and cryo-electron microscopy reconstructions combined with molecular data are beginning to reveal how these nano-structures are built. Topics covered in the course will include basic principles of virus structure and symmetry, capsid assembly, strategies for enclosing nucleic acid, proteins involved in entry and exit, and the life cycles of well understood pathogens such as HIV, influenza, polio, and Herpes. A review of cutting edge structural methods is also covered.
Watson and Crick noted that the size of a viral genome was insufficient to encode a protein large enough to encapsidate it and reasoned, therefore that a virus shell must be composed of multiple, but identical subunits. Today, high resolution structures of virus capsids reveal the basis of this genetic economy as a highly symmetrical structure, much like a geodesic dome composed of protein subunits. Crystallographic structures and cryo-electron microscopy reconstructions combined with molecular data are beginning to reveal how these nano-structures are built. Topics covered in the course will include basic principles of virus structure and symmetry, capsid assembly, strategies for enclosing nucleic acid, proteins involved in entry and exit, and the life cycles of well understood pathogens such as HIV, influenza, polio, and Herpes. A review of cutting edge structural methods is also covered.
