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课程概要
控制真核生物和原核生物基因组的维持,表达与演化的生化机制。7.28的授课内容与主要的阅读著作涵括以下主题:DNA的复制、DNA的修复、基因重组、基因表达、RNA加工、翻译。并且着重于实验设计的逻辑与数据的分析。上课方式包括讲课、指定阅读与分组讨论。写作作业(选择性的,参见下方)、习题(不计分)和复习课也对课程内容有帮助。
授课题目
复制(复制的酵素)
复制(复制叉)
复制(原核系统)
复制(真核系统)
染色质合成
DNA错误配对的修复
DNA修复II
双股断裂的修复、同源重组
同源重组的酵素
定点重组
减数分裂的重组
转位
转录:原核生物的机制与启动子
转录:真核生物的机制和启动子
转录:调控I
转录:调控II
RNA加工:稳定RNAs和RNA编辑
RNA加工:重要性、局部化等等
RNA加工:机制
翻译:基本机制I
翻译:基本机制II
翻译:调控
翻译后的调控
先修课程
7.03 遗传学
与
7.05普通生物化学
(可以接受同时进修7.05)
教科书
Lewin,
Genes VII
(选购)
上课时数
每周2节
每节2小时.
评分方式
评等
。每次考试将占同样的比重,因此每次测验将占你最后分数的33.3%
(选择性书写作业也会有得分,参见下方)
。
每次
测验
的内容原则上将会涵括自前次测验后新教的数据;然而本课程的设计是建立在之前教的技术和观念上,所以测验将反应对此的组织能力。除非有从医疗部门(或是医师)取得证明或是教务处的认可的理由,否则不能缺考。有理由的缺考你必须事先联络Bell教授或是Baker教授。如果期末考的排程有冲突请找Baker教授。
每次测验都会有涵括
测验资料
的习题。习题将不会计分,但是习题在深度和难度上会和测验中的题目相似。因此强烈建议习题要做(先不看答案!)。
课程的每三分之一,会指定一篇与课程题材相关的
研究论文
。每个人都应该阅读这些论文;这些论文会以论述的方式讨论,并且测验中也许会出现相关的题目。每篇论文我们都将会提出讨论问题。并且鼓励学生根据这些问题提出
小论文
(2页双倍行距)。论文将会根据答案的完整性、说明的清楚度与独创性评分,分数最高可到15分。
课程成绩
会只根据考试分数计算。完成的书写作业的分数会加到考试的总分上。总分将不变动的转换成相对的字母级别。因此书写作业的分数将视作额外的分数。
Course Overview
Detailed analysis of the biochemical mechanisms that control the maintenance, expression, and evolution of prokaryotic and eukaryotic genomes. Topics covered in lecture and readings of primary literature will include: gene regulation, DNA replication, genetic recombination, RNA processing, and translation. The logic of experimental design and data analysis will be emphasized. Presentations will include lectures, reading assignments and group discussions. Writing assignments (optional, see below), Problem Sets (ungraded) and review sessions also contribute to the course content.
Lecture Topics
Replication (Replication Enzymes)
Replication (Replication Fork)
Replication (Prok. System)
Replication (Euk. Systems)
Chromatin Assembly
DNA Mismatch Repair
DNA Repair II
DS Break Repair, Homologous Recombination
Homologous Recombination Enzymes
Site-specific Recombination
Meitoic Recombination
Transposition
Transcription: Prok. Machinery and Promoters
Transcription: Euk. Machinery and Promoters
Transcription: Regulation I
Transcription: Regulation II
RNA Processing: Stable RNAs and RNA Editing
RNA processing: Importance, Localization etc.
RNA Processing: Mechanism
Translation: Basic Machinery I
Translation: Basic Machinery II
Translation: Regulation
Post-translational Regulation
Prerequisites
7.03 Genetics
and
7.05 General Biochemistry
(simultaneous registration of 7.05 is acceptable)
Text
Lewin,
Genes VII
(Optional)
Lectures
每周2节
每节2小时.
Evaluation
Grading
for the course will be based on performance on the three scheduled exams. Each exam will be weighted equally, and therefore constitute 33.3% of your final grade
(optional writing assignments can also contribute, see below).
Material covered on each
exam
will be based principally on the new material presented since the previous exam; however, the course is designed to build on previously presented techniques and concepts and the exams will reflect this organization. Absence from an exam will be accepted only if an excuse is obtained from the Medical Department (or another physician) or with an excuse approved by the Dean's office. You must contact either Prof. Bell or Prof. Baker prior to the exam for an excused absence. If there is a conflict with the scheduled time of the final exam, see Prof. Baker.
There will be a
problem set
covering the material for each exam. These will not be graded, but the questions appearing on the problem sets will be very similar in depth and difficulty to those appearing on the exams. Therefore, working the problem sets (without reading the answers first!) is strongly recommended.
For each third of the course, one
research article
related to the material covered in lecture will be assigned. Everyone should read these papers; they will be discussed in recitation and related questions may appear on the exams. With each paper, we will also hand out a discussion question. Students are encouraged to write a
brief essay
(2 double spaced pages) in response to this question. Essays will receive up to 15 pts and will be graded based on the completeness of answer, clarity of explanation and originality.
Course grades
will be calculated based exclusively on exam scores. Points accumulated from completing the writing assignments will then be added to the exam totals. Grades will then be assigned without altering the point totals corresponding to each letter grade. Thus, the writing assignments will be treated as extra credit.
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